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ELISA方法被廣泛應用于各種抗原和抗體測定。但ELISA測定中影響因素較多，而且其操作中有一定的技術要求，在檢測過程中除正常反應外，有時常見到一些錯誤的結果。引起ELISA測定錯誤結果的原因主要有：①標本因素；②試劑因素；③操作因素。下面就一些常見ELISA操作過程中的問題一一分析。
1．樣品稀釋 酶聯免疫反應是一種敏感性很高的反應，如果血清不稀釋，不可避免會產生很強的非特異性反應，出現假陽性。因此，國外檢測血清抗體的試劑盒，均規定將血清稀釋至適當的倍數，以降低非特異性反應，使特異性的抗原抗體反應充分體現出來。一般產品的樣品稀釋倍數均通過大量試驗確定，以保證試驗的靈敏度和特異性。但是，有些用戶未能嚴格按使用說明書操作，如某些產品應取10μl樣品進行稀釋，個別用戶取5μl甚至1μl樣品進行稀釋，由于吸嘴上不可避免地沾有樣品以及微量移液器的精度不夠，因此造成樣品稀釋倍數不準確，檢測結果出現問題。
2．試劑盒平衡 試劑盒中所有試劑和板條均應在試驗前平衡至室溫（約25℃），一般需在室溫放置20～30分鐘以上。平衡時間太短會造成試劑混勻不夠，樣品孵育時間相對縮短，ELISA反應不夠充分。冬季室溫低，可將試劑盒置37℃溫箱20分鐘。
3．樣品和試劑的混勻 稀釋前、后的樣品必須充分混勻，所有試劑在加樣前也須搖勻，以保證試驗的均一性。
| MEXN-H0001 | 人α1酸性糖蛋白(α1-AGP)酶聯免疫吸附測定試劑盒 | Human α1-AGP (Alpha-1-Acid Glycoprotein) ELISA Kit |
| MEXN-H0002 | 人血管緊張素轉化酶 (ACE)酶聯免疫吸附測定試劑盒 | Human ACE (Angiotensin I Converting Enzyme) ELISA Kit |
| MEXN-H0003 | 人激活素A(ACVA)酶聯免疫吸附測定試劑盒 | Human ACVA (Activin A) ELISA Kit |
| MEXN-H0004 | 人脂聯素(ADP/Acrp30)酶聯免疫吸附測定試劑盒 | Human ADP/Acrp30 (Adiponectin) ELISA Kit |
| MEXN-H0005 | 人Agouti相關蛋白(AgRP)酶聯免疫吸附測定試劑盒 | Human AgRP (Agouti Related Protein) ELISA Kit |
| MEXN-H0006 | 人血管生成素1(ANG1)酶聯免疫吸附測定試劑盒 | Human ANG1 (Angiopoietin 1) ELISA Kit |
| MEXN-H0007 | 人血管生成素2(ANG2)酶聯免疫吸附測定試劑盒 | Human ANG2 (Angiopoietin 2) ELISA Kit |
| MEXN-H0008 | 人B細胞活化因子 (BAFF/CD257)酶聯免疫吸附測定試劑盒 | Human BAFF/CD257 (B-Cell Activating Factor) ELISA Kit |
| MEXN-H0009 | 人腦源性神經因子(BDNF)酶聯免疫吸附測定試劑盒 | Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit |
| MEXN-H0010 | 人骨成型蛋白2 (BMP-2)酶聯免疫吸附測定試劑盒 | Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit |
| MEXN-H0011 | 人骨成型蛋白4 (BMP-4)酶聯免疫吸附測定試劑盒 | Human BMP-4 (Bone Morphogenetic Protein 4) ELISA Kit |
| MEXN-H0012 | 人骨成型蛋白7 (BMP-7)酶聯免疫吸附測定試劑盒 | Human BMP-7 (Bone Morphogenetic Protein 7) ELISA Kit |
| MEXN-H0013 | 人上皮型鈣黏蛋白 (E-Cad)酶聯免疫吸附測定試劑盒 | Human E-Cad (E-Cadherin) ELISA Kit |
| MEXN-H0014 | 人碳酸酐酶IX (CA9)酶聯免疫吸附測定試劑盒 | Human CA9 (Carbonic Anhydrase IX) ELISA Kit |
| MEXN-H0015 | 人胱天蛋白酶1(CASP1)酶聯免疫吸附測定試劑盒 | Human CASP1 (Caspase 1) ELISA Kit |
| MEXN-H0016 | 人半胱天冬酶3(CASP3)酶聯免疫吸附測定試劑盒 | Human CASP3 (Caspase 3) ELISA Kit |
| MEXN-H0017 | 人組織蛋白酶B(CTSB)酶聯免疫吸附測定試劑盒 | Human CTSB (Cathepsin B) ELISA Kit |
| MEXN-H0018 | 人組織蛋白酶V(CTS5)酶聯免疫吸附測定試劑盒 | Human CTS5 (Cathepsin V) ELISA Kit |
| MEXN-H0019 | 人單核細胞趨化蛋白1(MCP-1/CCL2/MCAF)酶聯免疫吸附測定試劑盒 | Human MCP-1/MCAF (Monocyte Chemotactic Protein 1/Monocyte Chemotactic And Activating Factor) ELISA Kit |
| MEXN-H0020 | 人巨噬細胞炎性蛋白1α(MIP-1α/CCL3)酶聯免疫吸附測定試劑盒 | Human MIP-1α/CCL3 (Macrophage Inflammatory Protein 1α) ELISA Kit |
| MEXN-H0021 | 人巨噬細胞炎性蛋白1β(MIP-1β/CCL4)酶聯免疫吸附測定試劑盒 | Human MIP-1β/CCL4 (Macrophage Inflammatory Protein 1β) ELISA Kit |
| MEXN-H0022 | 人正常T細胞表達和分泌因子(RANTES/CCL5)酶聯免疫吸附測定試劑盒 | Human RANTES/CCL5 (Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit |
| MEXN-H0023 | 人單核細胞趨化蛋白3(MCP-3/CCL7)酶聯免疫吸附測定試劑盒 | Human MCP-3/CCL7 (Monocyte Chemotactic Protein 3) ELISA Kit |
| MEXN-H0024 | 人嗜酸粒細胞趨化因子1(ECF/Eotaxin 1/CCL11)酶聯免疫吸附測定試劑盒 | Human ECF/Eotaxin 1/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit |
| MEXN-H0025 | 人胸腺激活調節趨化因子(TARC/CCL17)酶聯免疫吸附測定試劑盒 | Human TARC/CCL17 (Thymus Activation Regulated Chemokine) ELISA Kit |
| MEXN-H0026 | 人巨噬細胞炎性蛋白3α(MIP-3α/CCL20)酶聯免疫吸附測定試劑盒 | Human MIP-3α/CCL20 (Macrophage Inflammatory Protein 3α) ELISA Kit |
| MEXN-H0027 | 人二級淋巴組織趨化因子(SLC/CCL21)酶聯免疫吸附測定試劑盒 | Human SLC/CCL21 (Secondary Lymphoid Tissue Chemokine) ELISA Kit |
| MEXN-H0028 | 人巨噬細胞來源的趨化因子(MDC/CCL22)酶聯免疫吸附測定試劑盒 | Human MDC/CCL22 (Macrophage Derived Chemokine) ELISA Kit |
| MEXN-H0029 | 人嗜酸粒細胞趨化因子2/髓樣前體細胞抑制因子2(MPIF2/CCL24/Eotaxin 2)酶聯免疫吸附測定試劑盒 | Human MPIF2/CCL24/Eotaxin 2 (Myeloid Progenitor Inhibitory Factor 2) ELISA Kit |
| MEXN-H0030 | 人嗜酸粒細胞趨化因子3(MIP4α/CCL26/Eotaxin 3)酶聯免疫吸附測定試劑盒 | Human MIP4α/CCL26/Eotaxin 3 (Macrophage Inflammatory Protein 4 Alpha) ELISA Kit |
| MEXN-H0031 | 人皮膚T細胞虜獲趨化因子(CTACK/CCL27)酶聯免疫吸附測定試劑盒 | Human CTACK/CCL27 (Cutaneous T-cell Attracting Chemokine) ELISA Kit |
| MEXN-H0032 | 人可溶性CD14分子(CD14)酶聯免疫吸附測定試劑盒 | Human CD14 (Soluble Cluster of Differentiation14) ELISA Kit |
| MEXN-H0033 | 人免疫球蛋白E Fc段受體Ⅱ(FcεRⅡ/CD23)酶聯免疫吸附測定試劑盒 | Human CD23/FcεRII(Receptor II for the Fc Region of Immunoglobulin E) ELISA Kit |
| MEXN-H0034 | 人白細胞分化抗原CD40配體(CD40L/TNFSF5)酶聯免疫吸附測定試劑盒 | Human CD40L/TNFSF5 (Cluster of Differentiation 40 Ligand) ELISA Kit |
| MEXN-H0035 | 人CD163分子(CD163)酶聯免疫吸附測定試劑盒 | Human CD163 (Cluster of Differentiation 163) ELISA Kit |
| MEXN-H0036 | 人軟骨糖蛋白39(GP39)酶聯免疫吸附測定試劑盒 | Human GP39 (Glycoprotein 39, Cartilage/Chitinase 3-Like 1) ELISA Kit |
| MEXN-H0037 | 人叢生蛋白(CLU)酶聯免疫吸附測定試劑盒 | Human CLU (Clusterin) ELISA Kit |
| MEXN-H0038 | 人睫狀神經營養因子(CNTF)酶聯免疫吸附測定試劑盒 | Human CNTF (Ciliary Neurotrophic Factor) ELISA Kit |
| MEXN-H0039 | 人組織因子/凝血因子(TF/CD142)酶聯免疫吸附測定試劑盒 | Human TF/CD142 (Tissue Factor/Coagulation Factor III) ELISA Kit |
| MEXN-H0040 | 人補體因子D(CFD)酶聯免疫吸附測定試劑盒 | Human CFD (Complement Factor D/Adipsin) ELISA Kit |
| MEXN-H0041 | 人Corin蛋白(CRN)酶聯免疫吸附測定試劑盒 | Human CRN (Corin) ELISA Kit |
| MEXN-H0042 | 人C反應蛋白(CRP)酶聯免疫吸附測定試劑盒 | Human CRP (C-Reactive Protein) ELISA Kit |
| MEXN-H0043 | 人趨化因子C-X3-C-基元配體1(CX3CL1)酶聯免疫吸附測定試劑盒 | Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1/Fractalkine) ELISA Kit |
| MEXN-H0044 | 人生長調節致癌基因α(GROα/CXCL1)酶聯免疫吸附測定試劑盒 | Human GROα/CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit |
| MEXN-H0045 | 人上皮中性粒細胞活化肽78(ENA-78/CXCL5)酶聯免疫吸附測定試劑盒 | Human ENA-78/CXCL5 (Epithelial Neutrophil Activating Peptide 78) ELISA Kit |
| MEXN-H0046 | 人粒細胞趨化蛋白2(GCP-2/CXCL6)酶聯免疫吸附測定試劑盒 | Human GCP-2/CXCL6 (Granulocyte Chemotactic Protein-2) ELISA Kit |
| MEXN-H0047 | 人白介素8 (IL-8)酶聯免疫吸附測定試劑盒 | Human IL-8 (Interleukin 8) ELISA Kit |
| MEXN-H0048 | 人抗載脂蛋白抗體(AAHA)酶聯免疫吸附測定試劑盒 | Human AAHA (Anti-Apolipoprotein Antibody) ELISA Kit |
| MEXN-H0049 | 人10kDa干擾素γ誘導蛋白(IP-10/CXCL10)酶聯免疫吸附測定試劑盒 | Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit |
| MEXN-H0050 | 人干擾素誘導T細胞α亞族趨化因子 (I-TαC/CXCL11)酶聯免疫吸附測定試劑盒 | Human I-TαC/CXCL11 (Interferon Inducible T-cell Alpha Chemoattractant) ELISA Kit |
| MEXN-H0051 | 人基質細胞衍生因子1(SDF-1/CXCL12)酶聯免疫吸附測定試劑盒 | Human SDF-1/CXCL12 (Stromal Cell Derived Factor 1) ELISA Kit |
| MEXN-H0052 | 人B-淋巴細胞趨化因子1(BLC1/CXCL13/BCA1)酶聯免疫吸附測定試劑盒 | Human CXCL13/BLC/BCA1 (B-Lymphocyte Chemoattractant 1) ELISA Kit |
| MEXN-H0053 | 人CXC趨化因子配體16 (CXCL16)酶聯免疫吸附測定試劑盒 | Human CXCL16 (Chemokine C-X-C-Motif Ligand 16) ELISA Kit |
| MEXN-H0054 | 人胱抑素C/半胱氨酸蛋白酶抑制劑3(Cys-C/CST3)酶聯免疫吸附測定試劑盒 | Human Cys-C (Cystatin C/Cystatin 3) ELISA Kit |
| MEXN-H0055 | 人細胞色素C(Cyt-C)酶聯免疫吸附測定試劑盒 | Human Cyt-C (Cytochrome C) ELISA Kit |
| MEXN-H0056 | 人Dickkopf相關蛋白1 (DKK1)酶聯免疫吸附測定試劑盒 | Human DKK1 (Dickkopf Related Protein 1) ELISA Kit |
| MEXN-H0057 | 人二肽基肽酶IV (DPP4/CD26)酶聯免疫吸附測定試劑盒 | Human DPP4/CD26 (Dipeptidyl Peptidase IV) ELISA Kit |
| MEXN-H0058 | 人表皮生長因子(EGF)酶聯免疫吸附測定試劑盒 | Human EGF (Epidermal Growth Factor) ELISA Kit |
| MEXN-H0059 | 人表皮生長因子受體(EGFR/ErbB1)酶聯免疫吸附測定試劑盒 | Human EGFR/ErbB1 (Epidermal Growth Factor Receptor) ELISA Kit |
| MEXN-H0060 | 人內分泌腺來源的血管內皮生長因子(EG-VEGF/PK1)酶聯免疫吸附測定試劑盒 | Human EG-VEGF/PK1 (Endocrine Gland Derived Vascular Endothelial Growth Factor)ELISA Kit |
| MEXN-H0061 | 人內皮糖蛋白(ENG/CD105)酶聯免疫吸附測定試劑盒 | Human ENG/CD105 (Endoglin) ELISA Kit |
| MEXN-H0062 | 人內皮抑制素(ES)酶聯免疫吸附測定試劑盒 | Human ES (Endostatin) ELISA Kit |
| MEXN-H0063 | 人內皮素1(ET-1)酶聯免疫吸附測定試劑盒 | Human ET-1 (Endothelin-1) ELISA Kit |
| MEXN-H0064 | 人內皮細胞蛋白C受體(EPCR)酶聯免疫吸附測定試劑盒 | Human EPCR (Endothelial Protein C Receptor) ELISA Kit |
| MEXN-H0065 | 人促紅細胞生成素(EPO)酶聯免疫吸附測定試劑盒 | Human EPO (Erythropoietin) ELISA Kit |
| MEXN-H0066 | 人凋亡相關因子(FAS/CD95)酶聯免疫吸附測定試劑盒 | Human FAS/CD95 (Factor Related Apoptosis) ELISA Kit |
| MEXN-H0067 | 人凋亡相關因子配體(FASL/TNFSF6)酶聯免疫吸附測定試劑盒 | Human FASL/TNFSF6 (Factor Related Apoptosis Ligand) ELISA Kit |
| MEXN-H0068 | 人脂肪酸結合蛋白1(FABP1/L-FABP)酶聯免疫吸附測定試劑盒 | Human FABP1/L-FABP (Fatty Acid Binding Protein 1) ELISA Kit |
| MEXN-H0069 | 人甲胎蛋白(αFP)酶聯免疫吸附測定試劑盒 | Human αFP (Alpha-Fetoprotein) ELISA Kit |
| MEXN-H0070 | 人酸性成纖維細胞生長因子(AFGF/FGF1)酶聯免疫吸附測定試劑盒 | Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) ELISA Kit |
| MEXN-H0071 | 人丙氨酸氨肽酶(AAP)酶聯免疫吸附測定試劑盒 | Human AAP (Alanine Aminopeptidase) ELISA Kit |
| MEXN-H0072 | 人成纖維細胞生長因子19(FGF19)酶聯免疫吸附測定試劑盒 | Human FGF19 (Fibroblast Growth Factor 19) ELISA Kit |
| MEXN-H0073 | 人成纖維細胞生長因子21(FGF21)酶聯免疫吸附測定試劑盒 | Human FGF21(Fibroblast Growth Factor 21) ELISA Kit |
| MEXN-H0074 | 人FMS樣酪氨酸激酶3配體(Flt3L)酶聯免疫吸附測定試劑盒 | Human Flt3L(FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit |
| MEXN-H0075 | 人卵泡抑素(FST)酶聯免疫吸附測定試劑盒 | Human FST (Follistatin) ELISA Kit |
| MEXN-H0076 | 人鐵調素(Hepc)酶聯免疫吸附測定試劑盒 | Human Hepc (Hepcidin) ELISA Kit |
| MEXN-H0077 | 人生長停滯特異性蛋白6(Gas6)酶聯免疫吸附測定試劑盒 | Human Gas6 (Growth Arrest Specific Protein 6) ELISA Kit |
| MEXN-H0078 | 人粒細胞集落刺激因子(G-CSF)酶聯免疫吸附測定試劑盒 | Human G-CSF (Granulocyte Colony Stimulating Factor 3) ELISA Kit |
| MEXN-H0079 | 人生長分化因子15(GDF15)酶聯免疫吸附測定試劑盒 | Human GDF15 (Growth Differentiation Factor 15) ELISA Kit |
| MEXN-H0080 | 人粒細胞巨噬細胞集落刺激因子(GMCSF/CSF2)酶聯免疫吸附測定試劑盒 | Human GMCSF/CSF2(Granulocyte Macrophage Colony Stimulating Factor 2) ELISA Kit |
| MEXN-H0081 | 人可溶性糖蛋白130(sgp130)酶聯免疫吸附測定試劑盒 | Human sgp130 (Soluble Glucoprotein 130) ELISA Kit |
| MEXN-H0082 | 人激活素AB(ACVAB)酶聯免疫吸附測定試劑盒 | Human ACVAB (Activin AB) ELISA Kit |
| MEXN-H0083 | 人肝細胞生長因子(HGF)酶聯免疫吸附測定試劑盒 | Human HGF (Hepatocyte Growth Factor) ELISA Kit |
| MEXN-H0084 | 人β干擾素(IFN-β)酶聯免疫吸附測定試劑盒 | Human IFN-β (Interferon Beta) ELISA Kit |
| MEXN-H0085 | 人胰島素樣生長因子1(IGF-1)酶聯免疫吸附測定試劑盒 | Human IGF-1 (Insulin-Like Growth Factor 1) ELISA Kit |
| MEXN-H0086 | 人胰島素樣生長因子結合蛋白3(IGFBP-3)酶聯免疫吸附測定試劑盒 | Human IGFBP-3 (Insulin Like Growth Factor Binding Protein 3) ELISA Kit |
| MEXN-H0087 | 人白介素1α(IL-1α)酶聯免疫吸附測定試劑盒 | Human IL-1α (Interleukin 1 Alpha) ELISA Kit |
| MEXN-H0088 | 人白介素1受體拮抗劑(IL-1ra/IL-1F3)酶聯免疫吸附測定試劑盒 | Human IL-1ra/IL-1F3 (Interleukin 1 Receptor Antagonist) ELISA Kit |
| MEXN-H0089 | 人白介素1受體II(IL-1R2)酶聯免疫吸附測定試劑盒 | Human IL-1R2 (Interleukin 1 Receptor Type II) ELISA Kit |
| MEXN-H0090 | 人前列腺特異性抗原(PSA/KLK3)酶聯免疫吸附測定試劑盒 | Human PSA/KLK3 (Prostate Specific Antigen/Kallikrein 3) ELISA Kit |
| MEXN-H0091 | 人成纖維細胞生長因子7(FGF7)酶聯免疫吸附測定試劑盒 | Human FGF7 (Fibroblast Growth Factor 7) ELISA Kit |
| MEXN-H0092 | 人瘦素受體(LEPR)酶聯免疫吸附測定試劑盒 | Human LEPR (Leptin Receptor) ELISA Kit |
| MEXN-H0093 | 人白血病抑制因子(LIF)酶聯免疫吸附測定試劑盒 | Human LIF (Leukemia Inhibitory Factor) ELISA Kit |
| MEXN-H0094 | 人腫瘤壞死因子配體超家族成員14(TNFSF14/LIGHT)酶聯免疫吸附測定試劑盒 | Human TNFSF14/LIGHT (Tumor Necrosis Factor Ligand Superfamily, Member 14)ELISA Kit |
| MEXN-H0095 | 人中性粒細胞明膠酶相關脂質運載蛋白(NGAL)酶聯免疫吸附測定試劑盒 | Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit |
| MEXN-H0096 | 人巨噬細胞集落刺激因子(MCSF)酶聯免疫吸附測定試劑盒 | Human MCSF (Macrophage Colony Stimulating Factor 1) ELISA Kit |
| MEXN-H0097 | 人基質金屬蛋白酶原1(Pro-MMP-1)酶聯免疫吸附測定試劑盒 | Human Pro-MMP-1 (pro-Matrix Metalloproteinase 1) ELISA Kit |
| MEXN-H0098 | 人白介素2(IL-2)酶聯免疫吸附測定試劑盒 | Human IL-2 (Interleukin 2) ELISA Kit |
| MEXN-H0099 | 人白介素3(IL-3)酶聯免疫吸附測定試劑盒 | Human IL-3(Interleukin 3) ELISA Kit |
| MEXN-H0100 | 人白介素4(IL-4)酶聯免疫吸附測定試劑盒 | Human IL-4 (Interleukin 4) ELISA Kit |
